Microbes are the most abundant and diverse group of organisms in nature, forming intricate and dynamic ecosystems that evolve over time. Yet, it is often difficult to untangle the myriad of complex interactions that occur in a heterogeneous microbial community. We are specifically interested in understanding the key principles that drive the formation, maintenance, and evolution of genomes within and across microbial populations. We apply new approaches in systems and synthetic biology to address important questions that lie at the intersection of genomics, evolution, and ecology. We utilize genome engineering, DNA synthesis, and next-generation sequencing methods to better understand genome evolution and population dynamics of a variety of model organisms and microbial communities under different environments. Furthermore, we aim to develop synthetic approaches in ecological engineering to manipulate disease-relevant microbial ecosystems such as those found on the human body and in the human gut towards ultimately improving human health.
Please visit my lab at: http://wanglab.c2b2.columbia.edu/
Yaung SJ, Deng L, Li N, Braff JL, Church GM, Bry L, Wang HH#
, Gerber GK#. Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics. Mol. Sys. Biol. 11:788 (2015).
Mee MT, Collins JJ, Church GM#, Wang HH#
. Syntrophic Exchange in Synthetic Microbial Communities. Proc. Natl. Acad. Sci. U.S.A. 111:E2149–E2156 (2014).
Esvelt KM, Wang HH
Genome-Scale Engineering for Systems and Synthetic Biology, Mol Sys Biol 9:641 (2013).
Mee M, Wang HH
Engineering ecosystems and synthetic ecologies. Mol BioSys 8:2470-2483, (2012). Wang HH
*, Kim HB*, Cong L, Jeong JH, Bang D, Church GM. Genome-scale Promoter Engineering by Co-Selection MAGE. Nat Methods 9: 591-593 (2012).
Carr PA*, Wang HH
*, Sterling B*, Isaacs FJ, Xu G, Kraal L, Bang D, Jacobson J, Church GM. Enhanced Multiplex Genome Engineering through Cooperative Oligonucleotide Co-selection. Nucleic Acids Res DOI: 10.1093/nar/gks455 (2012).Wang HH
, Church GM. Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineering. Methods Enzymol 498:409-26, (2011).
Isaacs FJ*, Carr PA*, Wang HH
*, Lajoie MJ, Sterling B, Kraal L, Tolonen AC, Gianoulis TA, Goodman DB, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM. Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science 333: 348-53 (2011).Wang HH
*, Isaacs FJ*, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM. Programming cells by multiplex genome engineering and accelerated evolution. Nature 460:894-8, (2009).
Honors and Awards
- Sloan Research Fellowship2015
- ONR YIP Award2014
- NSF CAREER Award2012
- Forbes 30 under 30 in Science2011-2016
- NIH Director’s Early Independence Award 2011-2013
- Wyss Technology Development Fellowship 2009
- Collegiate Inventors Competition Grand Prize, National Inventors Hall of Fame