Research Faculty

Address
630 West 168th Street
Room 15-401
New York, NY 10032


Phone: 212-305-6369
Fax:

lag3@columbia.edu
Education and Training
Ph.D. 1970 University of California
Lloyd Greene, Ph.D.
Professor of Pathology and Cell Biology
Research Summary

Neuronal differentiation

The overall goal of research in this laboratory is to understand the mechanisms whereby neuronal precursors differentiate into mature functional neurons. To this end, we use the rat PC12 pheochromocytoma cell line developed in this laboratory as a model system to study the mechanism of action of nerve growth factor (NGF) and the steps that lead to neuronal differentiation. Among current projects in the laboratory are those addressing the following questions: 1) What is the essential property of the high-affinity NGF receptor that permits it to mediate the functional activities of NGF and how is this receptor different from the low-affinity, non-functional NGF receptor? 2) What is the transductional mechanism by which NGF receptor occupancy leads to subsequent response? 3) What are the steps beyond immediate transduction that lead to NGF mechanisms? 4) What is the mechanism by which NGF regulates growth cone motility? What are the molecules involved in this effect? 5) What genes are regulated by NGF? By what pathways are they regulated? 6) What are the mechanism by which NGF promotes the initiation and regeneration of neurites; specifically, what is the role of specific cytoskeleton proteins in this process? 7) How do NGF and other neuronotrophic substances maintain cell survival? 8) How do such agents regulate cell proliferation?


Selected Publications

Please click the PubMed link below to view Dr. Greene's publcations:

Honors and Awards

1965 B.S. with general and special honors

1975-1979 Sloan Foundation Fellowship in the Neurosciences

1981-1986 Career Development Award from the Irma T. Hirschl Trust

1986-1993 Javits Award (NIH-NINDS)

1987 Medal of Excellence for Contributions to Neuroscience Research CNR (Italy)

1994 Adele Blank Award for Research in Neurodegenerative Disease

2000 Dean's Distinguished Lecturer in Basic Sciences, Columbia University

Keywords

apoptosis, neurogenetics, neuron, neurotrophic factor, JUN kinase, cyclin, cyclin dependent kinase, phosphorylation, protein structure function, protooncogene, transcription factor, PC12 cell, SDS polyacrylamide gel electrophoresis, laboratory rat, microar
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